Evaluation of radial growth of Lactarius volemus in semisolid medium
DOI:
https://doi.org/10.19230/jonnpr.3841Keywords:
Lactarius deliciosus, wild mushroom, macromycete, basidiomycete, basidiocarpAbstract
Objective. To evaluate the radial growth of Lactarius volemus in five semi-solid culture media in vitro.
Materials and methods. Fruitful bodies of L. volemus from the Sierra Norte of the state of Oaxaca, Mexico, were cultured in the laboratory in Potato Dextrose Agar Papa, Czapek-Dox Agar, Malt Extract Agar, Potato Sucrose Agar and Dextrose Saboraud Agar; using two seeding techniques. The morphological characteristics of colonies obtained from different samples of the fruiting body were evaluated, as well as the radial growth of each one.
Results. The evaluated colonial growth allowed to select a culture medium that meets the optimal conditions for the cultivation of Lactarius volemus in vitro. Not all samples used develop abundant growth: the sample from latex shows little growth.
Conclusions. With the evaluation of the radial growth of Lactarius volemus a direct reference to the growth cycle of this species is obtained; it is possible to identify the exponential and stationary phases but the conditions of the medium do not allow evaluating the phase of death due to dehydration and reduction of the agar.
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References
Ceroni A. Detección y evaluación de germoplasma celulítico en 136 cepas de hongos de suelo. Lima, Perú. Facultad de Ciencias. Universidad Nacional Agraria. 1988.
Garza F, García J & Castillo J. Macromicetos asociados al bosque de Quercus rysophylla en algunas localidades del centro del estado de Nuevo León. Revista Mexicana de Micología. 1985. 1: 423-37.
Guzmán G. Diversity and use of traditional Mexican medicinal fungi. A review. International Journal of Medicinal Mushrooms. 2008. 10:209-17.
Khan F & Islam M. Fungal Lectins: Current Molecular and Biochemical Perspectives. International Journal of Biological Chemistry. 2011. 5:1-20.
Cuesta J & Jiménez J. Ficha Micológica del género Lactarius. Asociación Micológica El Royo. El Royo, Soria, España [monografía en internet]. 2016. [Consultado el 29 de noviembre de 2019] Disponible en: http://www.amanitacesarea.com/lactarius.html
Sandoval JA. Aislamiento y caracterización de lectina de Lactarius volemus. Oaxaca de Juárez, Oaxaca, México. Unidad de Bioquímica e Inmunología, Instituto Tecnológico de Oaxaca. 2016.
García BLM.. Guía de Setas y Hongos de Navarra. Navarra, España. 2015 [Consultado el 17 de octubre de 2016]. Disponible en: http://guiahongosnavarra1garciabona.blogspot.mx/2015/02/lactarius-volemus-fr.html
Giollant M, Guillot J, Damez M, Dusser M, Didier P & Didier E. Research on the possible lnvolvement of the funga1 lectin in recognition between mushroom and spruce during the early stages of mycorrhizae formation. Plant Physiology. 1993. 101: 513-22.
Pereira G, Campos JL, Chávez D, Anabalón L & Arriagada C. Caracterización del crecimiento miceliar del hongo ectomicorrícico Lactarius aff. deliciosus y su simbiosis con plántulas de Pinus radiata. Quebracho. 2014. 22 (1-2):30-9.
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