Extraction of Stevia rebaudiana bertoni sweetener glycosides by supercritical fluid methods.
DOI:
https://doi.org/10.19230/jonnpr.1390Keywords:
Stevia, Supercritical Fluid, Co-solvent, Glycosides, SweetenersAbstract
Aim. The aim was to evaluate the supercritical carbon dioxide extraction method with and without the addition of co-solvent to the system (mixture water: ethanol) to obtain the glycosides from leaves of Stevia rebaudiana Bertoni.
Methods. A SFT-150 SFE / SFR model with CO2 as a fluid was used for the supercritical extraction. The variables studied were temperature, pressure, extraction time and the presence or absence of the co- solvent (water-ethanol mixture in a concentration of 70:30 v/v), incorporated in different proportions to determine the effect on yield. The amount of glycoside sweeteners was analyzed by High Performance Liquid Chromatography (HPLC).
Results. The pressure was the factor that favored the extraction, which was selective in obtaining Rebaudioside A with yields no greater than 2%. The inclusion of the co-solvent achieved an increase in yield to values of 2.9%
Conclusion. Supercritical CO2 individually and mixed with ethanol-water as a co-solvent was not efficient to extract Stevia rebaudiana stevioside sweeteners
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