Null bactericidal effect of ultraviolet radiation emitted by LEDs.
DOI:
https://doi.org/10.19230/jonnpr.2016.1.6.1083Keywords:
Ultraviolet Rays, Bactericidal, diode.Abstract
This research has aimed to assess the bactericidal effect of ultraviolet light emitted by LEDS on the growth on Petri dishes of microorganisms whose legal limits in foods have been established.
An electrically fed apparatus has been designed with precise timing and a camera to prevent light spillage, in which two ultraviolet radiation emission devices were connected by LED technology at different wavelengths: through an array of LEDS emitting at around
350nm, and a single specific emission LED at 280nm.
1000 cfu of E. Coli and S. aureus sown on PCA were used as prototypes of gram negative and positive bacteria, respectively, onto which ultraviolet light was radiated at different time intervals, by means of both devices, with the whole experiment being carried out in triplicate .
In none of the three series of treatments at the two wavelengths were reductions in microbial growth observed. The series of sowings on PCA were done on unseeded plates in order to be able to discard the likelihood of subsequent recontamination.
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